SOLVED: You are cloning a 1.595 kb insert into a 3.6kb vector and need to set up a ligation reaction. You will use 250ng of vector and a 10:1 insert:vector molar ratio in your ligation reaction. Your ligation reaction will have a 40ul total volume. The DNA samples and ligation reaction components are in your ice bucket. You will not perform the heating and cooling step of the protocol from class. You have measured the concentrations of each using the Qubit fluorometer. Samples were prepared with
SOLVED: You are cloning a 1.595 kb insert into a 3.6kb vector and nee…
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